Polymerase chain reaction in Helicobacter pylori infection in Progress.doc

 PAGE \* MERGEFORMAT 14 Polymerase chain reaction in Helicobacter pylori infection in Progress Keywords:: Helicobacter pylori infection by polymerase chain reaction Found that Helicobacter pylori (Helicobacter pylori, Hp) has been 10 years in the 10 years of Hp during the rapid progress in the study, from the cellular level, gradually went down to the molecular level. Now that the Hp is gastritis, peptic ulcer of the main risk factors, but also closely related with gastric cancer. Hp’s own spiral-shaped structure and the secretion of bacterial flagella toxin, VacA, urease, sticky protease, catalase, phospholipase, heat shock protein, low molecular weight chemotactic protein adhesin and so its induced disease-related. Diagnosis of Hp infection from traditional bacteriology, immunology, molecular biology tools developed to approach, especially through the analysis of Hp DNA, it was clearly part of the nucleotide sequence of Hp, which for the polymerase chain reaction (polymerase chain reaction, PCR) Hp created the conditions for the study. In this paper, in recent years, research related to PCR in the application of Hp are reviewed. 1 PCR principle PCR was developed in recent years as a special DNA fragment amplified in vitro techniques, the principle of many papers on it have been described in detail here only for description of each PCR amplification cycle includes three steps, the first step of a degeneration, at high temperatures to open the double-stranded target DNA; Step 2, at a lower temperature so that primer complementarity with the target DNA; Step 3 in the middle temperature, under the action of the DNA polymerase, primer extension according to the template DNA, a typical PCR, including 30 ~ 50 cycles, and so repeat the cycle, so that the target nucleotide was amplified geometrically amplified by the PCR operation, there are several points must be considered. ① Select primer length of 15 to 30 bases is appropriate, too short, poor specificity, to