Polygonum cuspidatum extract anti-leukemia effect of resveratrol and its possible molecular mechanism.doc

 PAGE \* MERGEFORMAT 19 Polygonum cuspidatum extract anti-leukemia effect of resveratrol and its possible molecular mechanism [ABSTRACT] Objective To investigate the resveratrol in vitro inhibition of leukemia cell and its mechanisms. MTT colorimetric assay method of cell growth inhibition rate, flow cytometry, HE staining and laser confocal microscope used to observe the group leukemic cell cycle and cell apoptosis, Western Blotting method cells of the expression of Bcl 2 protein, immunohistochemical determination of leukemic cell signal transduction molecule expression p STAT3 activity. Results resveratrol time dose-dependent manner inhibited 3 leukemia cell proliferation, induce apoptosis, cell cycle distribution, decreased tyrosine phosphorylation of STAT3 activity. Conclusions of resveratrol on leukemia cells from different sources all have strong anti-tumor activity, significantly reduced STAT3 tyrosine phosphorylation activity, and further used for clinical treatment of leukemia, the accumulation of the experimental basis. [Keywords:] resveratrol leukemia cells ABSTRACT: Objective To explore the anti leukemia effects of resveratrol on three leukemia cell lines HuT 78, Jurkat and L1210 in vivo. Methods MTT colorimetry was used to measure the tumor cell growth repression rate. The level of Bcl 2 was measured by Western blotting. The cell cycle of cells was analyzed by flow cytometry. The expression of p STAT3 was determined by immunohistochemistry. Results Our findings indicated that resveratrol could inhibit proliferation, induce apoptosis, and influence cell cycle of HuT 78, Jurkat and L1210 cells in a dose and time dependent manner and reduce the expression of p STAT3 in vitro. Conclusion Resveratrol might have a chemotherapeutic potential for leukemia of various sources and reduce the expression of p STAT3 which provides experiment evidence for its application in clinical treatment of leukemia. KEY WORDS: resveratrol; apoptosis; leukemia