Polysaccharide Extraction of Rhodiola rosea.doc

 PAGE \* MERGEFORMAT 12 Polysaccharide Extraction of Rhodiola rosea Author: Li Cheng, YANG Changqing, Zhu Caifeng, Ge Yan-Ru [Keywords:] Rhodiola;,, polysaccharide;,, orthogonal design;,, extraction Abstract: Objective To select the best extraction of Rhodiola polysaccharides. Methods filter orthogonal design optimal extraction of Rhodiola polysaccharide with a phenol - sulfuric acid colorimetric determination of its content. Results Rhodiola optimal extraction process of polysaccharide, with 10 times as much water, extract three times, 1 h / time. Rhodiola polysaccharide content measured for the 5.012 6%, the average recovery was 99.39%, RSD of 0.81% (n = 6). Conclusion The method is simple, accurate, sensitive, and suitable for the extraction and purification of polysaccharides Rhodiola rosea. Keywords:: Rhodiola; polysaccharide; orthogonal design; Extraction Study on the Optimum Extracting Method of Polysaccharide from Rhodioloside Abstract: ObjectiveTo select the optimum extracting method of polysaccharide from Rhodioloside. MethodsTo select the optimum extracting method by using orthogonal design and the determination of polysaccharide was taken by phenol-sulfuric acid method. ResultsThe optimum extract method of the polysaccharide was determined by orthogonal design: the solvent was water and the material was 10:1, extracted 3 times, each time was 1 hour.The polysaccharide’s content by the optimum extracting method was 5.012 6%, the average recovery was 99.39% (n = 6), relative standard deviation was 0.81%. ConclusionThis method was simple, accurate and had a good sensitivity, and was suitable for the extraction and determination of polysaccharide Rhodiola sachalinensis. Keywords:: Rhodiola sachalinensis; Polysaccharide; Orthogonal design; Extraction Rhodiola Rhodiola sachalinensis A. Bor. Yes Crassulaceae Rhodiola perennial herb, is contained in the ‘Changbai Mountain Botanical Chi’ and ‘Flora of China’ in Chinese medicine has a similar rightin