Portulaca flavonoids against hypoxia in mice and its mechanism.doc

 PAGE \* MERGEFORMAT 8 Portulaca flavonoids against hypoxia in mice and its mechanism Author: Dong Wei, Wang Wan-Yin, Yue Yi Tian, Li Min [Keywords:], Portulaca oleracea [Abstract] Objective: To screen anti-hypoxia effect purslane active ingredients, as well as filtering out the mechanism of the active ingredients for research. Methods: purslane screening active ingredients of anti-hypoxia effect: The 70 mice were randomly divided into groups of total flavonoids, total alkaloid group, polysaccharide group, acidic component group, basic ingredients group ginsenosides group (positive control) and the saline group (negative control), 10 in each group. Mice in each group to be the appropriate drug gavage 1 week after exposure to hypoxia survival time of experiment, filter out the anti-hypoxia effect of Portulaca oleracea active ingredients. Portulaca mechanism of flavonoids against hypoxia: The 90 mice were randomly divided into groups of total flavonoids, ginsenosides group and saline group, each group was further divided into three sub-groups, namely, 12,24 and 36 of hypoxic exposure h group, n = 10. The mice were placed in nitrogen-oxygen mixture (10% O2 +90% N2) chamber 12,24, and 36h, respectively, after hypoxic exposure were measured red blood cell count, hemoglobin content, blood cells, hematocrit, plasma erythropoietin (erythropoietin, EPO) as well as the kidney and the expression level of the cerebral cortex EPOmRNA. Results: Compared with saline group, Portulaca oleracea flavonoids in mice significantly prolonged survival time of hypoxic exposure; Portulaca flavonoids in mice of different periods of renal hypoxia and cerebral cortex the expression of EPO mRNA levels significantly increased, plasma EPO , red blood cell count and hemoglobin levels have also increased significantly. Conclusion: The flavonoids are anti-hypoxia effect of Portulaca oleracea active ingredients, which prolong the survival time of hypoxic mechanism may promote the expression