Pretreatment on different frozen allograft nerve regeneration in rats.doc

 PAGE \* MERGEFORMAT 18 Pretreatment on different frozen allograft nerve regeneration in rats [Abstract] Objective To observe the freezing temperatures preserved nerve allograft repair on the effect of peripheral nerve defects. Methods 96 male rats were the same species, of which 24 is derived group, 72 were randomly divided into control group (A, B group and experimental group (C, D, E, F group, 12 in each group. A group of fresh nerve autograft group, B group fresh injection group, C, D, E and F groups, respectively at 4 , -50 , -80 -196 and kept for 3 weeks glycerol nerve group. After 2 weeks, observed by immunohistochemistry, after 9,16 weeks gross observation, light microscopy inspection, electrophysiological determination. Results After 2 weeks, B group transplanted apparent in T lymphocyte infiltration, C, D, E group, followed by, A group and F group no T lymphocytes. after 9 weeks and 16 weeks, from the evaluation of nerve regeneration see: A group of nerve regeneration was the best, F group, followed by, C, D, E group again, B group is the worst. Conclusions In the frozen group, group of nerve regeneration to -196 best, close to the fresh autologous nerve transplantation group. [Keywords:] low-temperature pretreatment of nerve graft nerve Abstract: Objective To investigate the regeneration of peripheral nerve allografts stored in the glycerol of different temperature.Methods A total of 96 male Wistar rats, were randomly divided into 7 groups.Group A and B were the control groups, each of which included 12 rats ; all underwent fresh femoral nerve autograft and allograft transplantation respectively.Twenty four rats in group G were sacrificed to obtain 48 femoral nerves, which were randomly divided into 4 groups, 12 nerves in each group, and were stored in glycerol for 3 weeks at 4 , -50 , -80 and -196 respectively.After storage for 3 weeks, 4 12 nerves in each temperature group were randomly transplanted into 4 12 rats in target rats