Production of a viral asthma and Evaluation Methods.docVIP

 PAGE \* MERGEFORMAT 18 Production of a viral asthma and Evaluation Methods [Abstract] Objective To evaluate the use of respiratory syncytial virus (RSV) induced by chicken egg albumin (OVA) sensitization, establish a mouse model of acute toxicity of the method and effect of asthma. Methods BALB / c mice, 100 were randomly divided into the phosphate buffer (PBS / PBS) control group, PBS / RSV groups, RSV / RSV group, OVA / PBS group and OVA / RSV group 5 group. application of multi-point injection of OVA sensitized, OVA inhalation of intranasally with RSV stimulate viral replication asthma model. acute attack of asthma symptoms in mice, determination of animal depiction acetylcholine box (Ach) stimulated conditions, airway responsiveness (expressed in lung resistance RL), bronchoalveolar lavage fluid (BALF) for Cell Count, HE staining of lung tissue pathology. Results 45,135,405 g / mL Ach fires, and other groups, OVA / RSV group were significantly increased RL (P all lt;0.01), BALF of inflammatory cells counted and compared with other groups, OVA / RSV group total cells, lymphocytes, eosinophils, neutrophils were significantly increased (P all lt;0.01), OVA / RSV group, lung tissue lesions is more important than the other groups (P both lt;0.01). RSV induction of OVA sensitized mice with acute viral methods to establish the asthmatic model is successful. [Keywords:] respiratory syncytial virus in acute asthma and evaluation of modeling methods ABSTRACT: Objective To reproduce a type of acute viral asthma model in ovalbumin (OVA) sensitized BALB / c mice by induction with respiratory syncytial virus (RSV). Methods One hundred mice were randomly divided into five groups: PBS / PBS control group, PBS / RSV group, RSV / RSV group, OVA / PBS group and OVA / RSV group. The asthma model was reproduced by sensitization with multipoint intraperitoneal injection of OVA, followed by repeated inhalation of OVA combined with repeated intranasal instillation of