Primary liver cancer in patients with p16 and the DAPK gene promoter methylation.docVIP

 PAGE \* MERGEFORMAT 3 Primary liver cancer in patients with p16 and the DAPK gene promoter methylation [Abstract] Objective To study the serum of patients with primary liver cancer gene p16 and DAPK promoter methylation status and clinical significance of the changes. Methods Methylation-specific PCR technique to detect 64 cases of PLC in patients with p16 gene and DAPK gene promoter methylation, and analysis of the relationship between the clinical and pathological data. The results of PLC in patients with p16 gene and DAPK gene methylation detection rates were 76.6% (49/64) and 40.6% (26/64), while the normal control group and benign liver disease, serum levels were not detected in p16 gene and DAPK gene methylation; p16 gene and DAPK gene methylation detection rate of HBsAg, stage and metastasis status had no significant relationship, are associated with the AFP. Conclusion p16 gene and DAPK gene promoter methylation abnormalities involved in the occurrence and development of PLC, PLC and can serve as an early diagnosis is one of the molecular marker. [Keywords:] primary liver cancer p16 gene DAPK gene methylation in serum DNA 0 Introduction p16 gene is an important tumor suppressor gene [1], involved in cell cycle regulation, control of cell growth and differentiation. Death-associated protein kinase (death  associated protein kinase, DAPK) gene is a calmodulin regulated serine / threonine protein kinase, is a positive regulator of apoptosis, one of [2,3], has been found in existence of a variety of tumor tissue p16 and DAPK gene promoter methylation, suggesting that the methylation of these genes may be related to the occurrence of tumor development. In this paper, methylation-specific PCR (MSP) to detect primary liver cancer (PLC) in patients with circulating nucleic acids in DAPK gene, p16 gene methylation status, and to explore the serum DAPK gene, p16 gene methylation in clinical significance. 1 Data and methods 1.1 The subjects were 64 case