Progress in detection of platelet aggregation - Determination of shear-induced platelet aggregation.docVIP

 PAGE \* MERGEFORMAT 8 Progress in detection of platelet aggregation - Determination of shear-induced platelet aggregation Keywords: Shear-induced platelet aggregation test Platelet aggregation function in physiological hemostasis and pathological thrombosis account for an important position. The platelet aggregation test started in 60 years, originally developed by Born and glass Turbidity determination [1]. This method is mainly used for determination of platelet aggregation induced by various agents in the low shear-rate-induced platelet aggregation. In recent years, shear-induced platelet aggregation (Shear-induced platelet aggregation, SIPA) under medical attention, this article will introduce the principles and applications. 1 Principle of Measurement Is generally believed that begins with a variety of platelet aggregation induced by agents and platelet membrane receptor interaction. This interaction to pass through the membrane and activated platelets, so that the surface of another membrane receptor glycoprotein IIb / IIIa (glucoprotein IIb / IIIa, GPIIb / IIIa) activation, and then with the combination of plasma fibrinogen, platelet aggregation intermediary . This typical aggregation of commonly used instrument to evaluate platelet aggregation. However, recent research found that the role of platelets by high shear stress in the absence of inducing agents also have a case of irreversible aggregation. High-shear and low shear-induced platelet aggregation are different, and its aggregation of different ligands. In the conventional low-shear environment, the gathering took place is determined by plasma fibrinogen intermediaries; and high shear environment, the aggregation is mediated vWF. vWF in the role of high shear environment, with its unique molecular structure. Under the action of high shear stress, vWF extending its huge filament, which mostly repeated polymer sub-units of a multivalent form of the possibility of combining in the platelet rece