Progress in immune-PCR technique.docVIP

 PAGE \* MERGEFORMAT 14 Progress in immune-PCR technique Abstract: Immune-PCR, a combination of antigen-antibody reaction specificity and high sensitivity of PCR is a highly sensitive antigen detection techniques, and suitable for a variety of micro-antigen detection. Fluorescent tags, enzyme tags and radioisotope marking the three monoclonal antibody technology is the immunochemistry, immunology and molecular biology of the most widely used means of conventional antigen detection with high sensitivity. However, in early cancer antigen and some neuropeptides such as a trace amount of antigen detection, the fluorescent marker and enzyme markers lack adequate sensitivity. Although the sensitivity of radioisotope labeling technique can achieve 1ng/ml, but in practice due to the need of special equipment and security, thus limiting its wide application in the actual process. In 1992, Sano [1] and others will immunoassay technology and PCR combined to create a new highly sensitive antigen detection techniques, namely the immune-PCR (Immuno-PCR). It appears to solve the above-mentioned three kinds of monoclonal antibody technology shortcomings. Is well known, PCR technology since its inception in 1985, after 10 years of development, has become the conventional laboratory techniques of modern molecular biology is also an indispensable means of research, is an extremely sensitive amplification system. The immune-PCR technique is highly sensitive nature of the use of PCR to amplify the specificity of antigen-antibody reaction, so that the experiment can be only hundreds of antigen detection, even in theory, can detect a limited number of antigens. This sensitivity of the immune detection technology has reached a new height. A basic principle of the immune-PCR Immune-PCR mainly consists of two parts. The first part is similar to the normal enzyme-linked immunosorbent assay (ELISA) of antigen-antibody reaction. The second part of the conventional PCR amplific