PSK vitamin E against CCl4 liver injury in mice studies.docVIP

PSK vitamin E against CCl4 liver injury in mice studies On: Sun Micro Zhang Hongmei Zhao Jie Tang set [Abstract] Objective To observe the PSK (CVP) and the combined use of CVP and VE CCl4 hepatic injury, hepatic injury of the role of CVP. METHODS 4 months 60 Kunming mice were randomly divided into control group , in the model, CVP protection group fed high and low dose (300,150 mg), CVP and protection group VE (VE 250 mg +150 mg CVP, VE 250 mg +300 mg CVP), n = 10. with CVP irrigation Canadian feed the stomach VE, keeping 7 d, the last gavage 2 h, the normal control group injected cooking oil solution, the other groups were injected intraperitoneally 0.15% CCl4 solution blend oil (10 ml / kg body weight), 24 h after ocular blood mice were killed and liver removed hepatosomatic index calculated after weighing liver homogenate; determination of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver homogenate superoxide dismutase (SOD) activity and malondialdehyde (MDA), glutathione (GSH) content. Results Compared with model group, CVP reduced hepatosomatic index and serum ALT and AST levels (P lt;0.05, P lt;0.01); increased SOD activity and GSH content (P lt;0.01), decreased MDA content (P lt;0.001), and a synergistic effect in combination VE. Conclusion CVP reduces experimental liver injury caused by inflammatory infiltration, increased SOD activity and GSH, content, speed up the free radical scavenging, on experimental liver injury has a protective effect, combined with the synergistic effect of VE. [Keywords:] PSK; vitamin E; liver injury; protection PSK (Coriolus versciclor polysaccharides CVP) with a variety of biological activity, can improve the antioxidant enzyme activity of free radicals and inhibit oxidative stress and antagonism (1,2); vitamin E (VE) is a classic anti- Oxidizing substances (3). the various causes of liver injury and oxidative stress are closely related, but the CVP on the mechanism of liver injury, a