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Pseudomonas aeruginosa multi-epitope DNA vaccine and its biological function of.doc

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Pseudomonas aeruginosa multi-epitope DNA vaccine and its biological function of

Pseudomonas aeruginosa multi-epitope DNA vaccine and its biological function of : Chen, Su Zhaoliang, Liuying Zhao, Wang Shengjun, Pengsu Fang, Li Yazhen, Shaoqi Xiang Xu of the River [Abstract] Objective: To construct P. aeruginosa (Pseudomonas aeruginosa, PA MyD88 multi-epitope DNA vaccine and its immunogenicity study. Methods: The PA outer membrane protein F (OprF three neutralizing B cell epitopes common spaces and a foreign T cell epitope in series, using overlapping PCR synthesized DNA, and inserted before the sequence of tissue plasminogen (tPA signal peptide gene, and this MyD88 gene fragments obtained were cloned into the pIRES vector plasmid pIRES tPA OprF: MyD88, and the plasmid-Balb / c mice determined by ELISA specific antibody levels. tracheal inoculation of PA, the PA lung tissue bacterial counts. Results: We successfully constructed the eukaryotic plasmid pIRES tPA OprF: MyD88, mice immunized with the plasmid, can stimulate the body to produce highly specific antibodies, the trachea after PA inoculation, immunized mice lung homogenate bacterial counts were significantly lower than other control group. CONCLUSION: The constructed There MyD88 gene recombinant PA epitope DNA vaccine, inoculated mice induced specific immune response after, resulting in better anti-Pseudomonas aeruginosa infection in immune protection. [Keywords:] Pseudomonas aeruginosa outer membrane protein F, MyD88, epitope vaccine, immunogenicity [Abstract] Objective: To construct the Pseudomonas aeruginosa multiepitope MyD88 gene vaccine and to study its immunogenicity in Balb / c mices. Methods: The epigene was designed and synthesized, containing three B cell epitopes of OprF and one foreign ‘promiscuous’T cell epitope by overlap extension PCR, and tPA signal encoding sequence was amplified by PCR method and then was inserted to the 5’terminus of the epigene to construct tPA OprF DNA fragment. The DNA fragment and MyD88 gene were cloned into expression vector pIRES to