Qinggan Huoxue Fang and its Ingredients on alcohol-induced liver injury induced by tumor necrosis factor-α expression in.doc

 PAGE \* MERGEFORMAT 13 Qinggan Huoxue Fang and its Ingredients on alcohol-induced liver injury induced by tumor necrosis factor-α expression in Author: Wu Tao, Liutao, Pei-Yong Zheng, Lian-Jun Xing, Ji-ray [Abstract] Objective: To observe the Qinggan Huoxue Party and its Ingredients on alcohol-induced liver injury (alcoholic liver injury, ALI) in rat liver tumor necrosis factor-α (tumor necrosis factor  α , TNF  α ) mRNA expression and plasma TNF  α in the impact. Methods: In addition to the blank group and carbon tetrachloride (carbon tetrachloride, CCl4) group, the 80 male Wistar rats were copied ALI model of complex factors. Modeling 4 weeks after rats were randomly divided into model group, Qinggan side group, activating blood group and Qingan Huoxue Fang Fang group. 3 to the corresponding drug treatment group fed. Gavage 2 weeks, serum alanine aminotransferase (alanine aminotransferase, ALT), aspartate aminotransferase (aspartate aminotransferase, AST) activity; using enzyme-linked immunosorbent assay determination of plasma TNF  α content; specimens from the liver specimens  hematoxylin eosin stain; reverse transcription-polymerase chain reaction for detection of liver tissue TNF  α mRNA expression. Results: Liver Huoxue parties and their split side ALI in rats can be significantly improved liver steatosis and liver inflammation level is better than Qinggan Qinggan Huoxue Fang Fang. Liver blood circulation model can only significantly reduce the serum ALT activity; Qinggan side, promoting blood circulation side and Qinggan Huoxue side ALI in rats can be significantly reduced serum AST activity between the two groups was no significant difference. Huoxue side and Liver blood circulation can only significantly reduce the model of rat liver tissue TNF  α mRNA expression and plasma TNF  α level, Qinggan side is not obvious. Conclusion: Liver Huoxue side and blood circulation in Treating mechanism of ALI may be related to reduced prod