Qinghuozhimai HPLC determination of andrographolide in cereals the content of dehydroandrographolide.doc

 PAGE \* MERGEFORMAT 19 Qinghuozhimai HPLC determination of andrographolide in cereals the content of dehydroandrographolide ZHAO Bao-hua, He Xiuxia, Li-Zhen Xu *, Shi-Lin Yang 【Abstract】 Objective To establish the right Qinghuozhimai cereals andrographolide and dehydroandrographolide HPLC determination method. Method of external standard method, DiamonsilTMC18 column (150mm × 4.6mm, 5μm); mobile phase: methanol -0.4% acetic acid solution (46:64); detection wavelength of 225nm and 250nm; flow 1ml/min. Results Andrographolide linear range of 0.112 ~ 0.391μg (r = 0.9997), the average recovery was 99.8%, RSD = 1.8% (n = 5); dehydroandrographolide linear range of 0.221 ~ 0.773μg ( r = 0.9996), the average recovery was 98.7%, RSD = 0.5% (n = 5). Conclusion The method is accurate, sensitive and can be used as cereal in Qinghuozhimai dehydroandrographolide andrographolide and quantitative analysis. 【Key Words】 tablets; andrographolide; dehydroandrographolide; high performance liquid chromatography 【Abstract】 Objective To determine the content of andrographolide, dehydroandrographolide in Qinghuozhimai tablets by HPLC. Methods An external standard method by HPLC with DiamonsilTMC18 column as fixed phase and methanol-0.4% HAC (46:64) as mobile phase was adopted. The detection wavelength was 225nm, 250nm and the flow rate was 1.0ml/min.Results The linear range for andrographolide was 0.112 ~ 0.391μg (r = 0.9997). The average recovery of tablets sample was 99.8% and RSD was 1.8% (n = 5 ); the linear range for dehydroandrographolide was 0.221 ~ 0.773μg (r = 0.9996). The average recovery of tablets sample was 98.7% and RSD was 0.5% (n = 5). Conclusion The method is simple and accurate, which can be adoptable for quantitative analysis of andrographolide, dehydroandrographolide of Qinghuozhimai tablets. 【Keywords:】 tablets; andrographolide; dehydroandrographolide; HPLC Qinghuozhimai cereals [1] is a CSC [2], gardenia,