Qingkailing HPLC fingerprint of different formulations Comparison.doc

 PAGE \* MERGEFORMAT 5 Qingkailing HPLC fingerprint of different formulations Comparison Author: Zhang Miao Yuan, Yao Jincheng, ZHAO Xu-yuan, HU collar [Keywords:] Qing Kai Ling;,, fingerprinting;,, high-performance liquid chromatography Abstract: Objective To compare the different preparations Qingkailing high-performance liquid chromatographic fingerprint. Methods NUDEOSILC18 column (4.6 mm * 250 mm, 5 μ m), mobile phase (A) methanol (B) 0.5% acetic acid gradient elution, running time 65 min, detection wavelength 255 nm, measured Qingkailing injection fingerprint map. Results are baicalin as a reference peak, reached the peaks of the relative retention time and relative peak area. Conclusion Qingkailing fingerprints of different preparations vary widely, suggesting that the production process may be different composition and content of their greater impact. Keywords:: Qing Kai Ling; fingerprinting; high performance liquid chromatography Abstract: ObjectiveTo compare the fingerprint chromatogram of different preparations of Qingkailing Compound. MethodsHPLC with NUDEOSIL-C18 column was used, the mobile phase was (a) methanol, (b) 0.5% acetate buffer and the detection wavelength was at 255nm.ResultsPeaks were indicated on the HPLCfingerprint of different preparations of Qingkailing Compound. The relative retention time and relative peak area were obtained with itself peak.ConclusionThe fingerprint chromatogram of different preparations of Qingkailing Compound are noticeably different, which indicates different manufacture technology affects ingredients and contents of Qingkailing preparations. Keywords:: Qingkailing Compound; Fingerprint chromatogram; HPLC Qingkailing set of square due to the treatment of acute fever of traditional Chinese medicine prescriptions ‘febrile disease Sambo’ in the Angongniuhuang Pill, from the acid, HDCA, buffalo horn powder, pearl powder, honeysuckle extract, skullcap glycosides, gardenia, Banlangen etc., so far has been f