Qingtiankui active site in vitro anti-tumor effect of.doc

 PAGE \* MERGEFORMAT 15 Qingtiankui active site in vitro anti-tumor effect of Author: Zhongzhen Guo Yuan Ye Fei Zhou Han-Shen Zhen LIANG Chen Yan Yanyuan [Abstract] Objective To determine the Guangxi specialty medicine Qingtiankui vitro anti-tumor effect of the active site. Methods Qingtiankui Preparation of extracts used mainly solvent method, the whole plant Qingtiankui first with 95% ethanol extraction, obtained extract further with petroleum ether, ethyl acetate, butanol, methanol followed by extraction, are polar by small to large four extracts. Activity screening method using MTT experiments, observations Qingtiankui extract on seven kinds of tumor cell growth inhibition. Results Qingtiankui parts petroleum ether and ethyl acetate has a certain anti-tumor effects in vitro. Conclusion For the first time Qingtiankui of petroleum ether and ethyl acetate part of an effective anti-tumor effect in vitro site. [Keywords:] Qingtiankui vitro anti-tumor effects of the active site Abstract: ObjectiveTo determine the active fraction with anticancer effect in vitro from Nervilia foadii (Hance) Schltr.MethodsThe samples were prepared by solvent extraction. The whole Nervilia foadii (Hance) Schltr. Was extracted with 95% ethanol at first, and then the ethanolic extract was separated with petroleum ether, ethyl acetate, n-Butyl alcohol and methanol in turn to get four fractions from small to large in polarity. The MTT experiment was made for active screening. The inhibiting influences of extracts from Nervilia foadii (Hance) Schltr . on growth of seven kinds of human tumor cells were observed. ResultsPetroleum ether extract and ethyl acetate extract both had some anticancer effects in vitro. ConclusionIt is the first time that the petroleum ether extract and ethyl acetate extract of Nervilia foadii (Hance) Schltr. are proved to be the effective anticancer fractions in vitro. Keywords:: Nervilia fordii (Hance) Schltr., Anticancer effect in vitro, Active fraction Qin