Rabbit anular puncture disc degeneration model induced by the histological and imaging analysis.doc

 PAGE \* MERGEFORMAT 15 Rabbit anular puncture disc degeneration model induced by the histological and imaging analysis Of: Yang Xiao Ning, Sun Tianwei, Wang Qiming Sun Yigong, Cao Lei [Abstract] Objective To investigate the puncture disc degeneration model induced by a viable and scientific. Methods 18 New Zealand white rabbits, the right side of the extraperitoneal approach with a curved vascular clamp holding 16G needle piercing the L3-4 accuracy , L4-5, L5-6 intervertebral disc annulus, the depth of control in 5 mm. at 4,8,12 weeks before and after surgery on the disc after the model of the disc and the control group (in L1-2, L2- 3, L6-7 as a control) line of computer X-ray photography (computed radiography, CR), MRI examination, and histological observation. results from the surgery after 4 to 12 weeks, after the model of the disc height index (disc height index , DHI) showed a decreasing trend, MRI T2WI signal continued to show weakening trend (P lt;0.01); 4,8 weeks, histological observation that nucleus pulposus cells decreased gradually in the model, nucleus pulposus cells were almost 12 weeks group was fibrous cartilage tissue replacement, fiber ring arranged irregularly, with ill-defined nucleus. Conclusion puncture method can induce a slow degeneration of rabbit intervertebral disc for the clinical study of intervertebral disc degeneration in depth to provide a practical animal model. [Keywords:] disc degeneration; puncture; rabbit; Imaging Abstract: Objective To investigate the feasibility and scientificalness of establishing an animal model of intervertebral disc degeneration induced by needle puncture in the anulus fibrosus. Methods 18 New Zealand white rabbits under anesthesia underwent puncture of the L3-4, L4-5 and L5-6 lumbar intervertebral discs by 16-gauge needle at a depth of about 5 mm in the right anterolateral anulus fibrosus. Computed radiography (CR), magnetic resonance imaging scans (MRI), and histological observations of