Rabbit bone marrow stromal stem cells and cartilage cells were cultured cartilage in vivo experimental observations.doc

 PAGE \* MERGEFORMAT 13 Rabbit bone marrow stromal stem cells and cartilage cells were cultured cartilage in vivo experimental observations Authors: Yun-Feng Ni, Xiao-Fei Li, Liu Yuan, mine warfare Jun, Lu Qiang [Keywords:] chondrocyte Experimental study on chondrogenesis by coculture of rabbit mesenchymal stem cells and chondrocytes [Abstract] AIM: To explore the feasibility of in vivo chondrogenesis by coculture of mesenchymal stem cells (MSCs) and chondrocytes. METHODS: Rabbit MSCs and chondrocytes were in vitro isolated, cultured and expanded respectively and then were mixed at a ratio of 3: 1 (MSCs: chondrocytes). The mixed cells were seeded onto PGA scaffold at the ultimate concentration of 6.0 * 1010 / L as coculture group. MSCs and chondrocytes of the same ultimate concentration were seeded respectively onto the scaffold as negative control group (MSCs group ) and positive control group (chondrocyte group). Then, the cellscaffold constructs were transplanted into subcutaneous tissue of adult rabbits. All specimens were harvested after in vivo culture for 8 weeks. Gross observation and histology were used to evaluate the new cartilage. RESULTS: Cells in all groups had a fine adhesion to the scaffold. In both coculture group and positive control group, the cellscaffold constructs could maintain the original size and shape during in vivo culture and formed homogenous mature cartilage after 8 weeks of in vivo culture. Furthermore, the neocartilages in both groups were similar to each other in gross appearance and histological features. In negative control group, the cartilage was not formed but the connective tissue. CONCLUSION: Chondrocytes can provide a chondrogenic microenvironment to induce a chondrogenic differentiation of MSCs and thus promote the chondrogenesis of MSCs in vivo. [Keywords] chondrocytes; MSCs; coculture; PGA [Abstract] Objective: To investigate the bone marrow stromal stem cells (MSCs) and cartilage cells in mixed culture the f