Rabbit bone marrow stromal cells in vitro and biological activity of.doc

 PAGE \* MERGEFORMAT 18 Rabbit bone marrow stromal cells in vitro and biological activity of Of: Zhang Chen, Kun-Zheng, Qiang-hui, when Zhibin, party Xiao Qian, Huang Xianghui [Abstract] Objective isolation and identification of rabbit bone marrow stromal cells (BMSCs) and explore the differentiation potential. Methods density gradient centrifugation combined adherence screening methods, in vitro isolation and culture of rabbit bone marrow-derived mesenchymal stem cells to develop during the inverted microscope The biological characteristics were observed, HE staining and CD34, CD44 immunohistochemical staining cytological identification of the line, the application in vitro osteogenic differentiation and adipogenic testing its biological activity. The results were successfully isolated and cultured in vitro rabbit BMSCs, HE staining showed that cells good homogeneity, CD44 expression strongly positive, CD34 negative. BMSCs induced bone showed strong osteogenic activity, ALP staining, showed adipogenic BMSCs have strong adipogenic activity, oil red “O” fat staining. Conclusions BMSCs in vitro multilineage differentiation potential capacity. [Keywords:] bone marrow stromal cells, cell culture, differentiation ABSTRACT: Objective To observe the effect of the in vitro isolation, culture and identification of rabbit bone marrow derived mesenchymal stem cells (BMSCs) and explore the differentiation potentials of BMSCs. Methods BMSCs were isolated and purified by density gradient centrifugation combined with attachment culture method . The biological characteristics of BMSCs were observed under an inverted microscope. The BMSCs were identified with HE staining and CD34/CD44 immunohistochemical staining. The biological activity of BMSCs was detected by osteogenic induction and steatogenic induction. Results BMSCs were isolated successfully in vitro and had good homogenicity. Immunohistochemical staining of CD34/CD44 showed strong positive expression of CD4