Rabbit experimental subarachnoid hemorrhage in the ultrastructure of blood vessels and pathological features and dynamic changes of.doc

 PAGE \* MERGEFORMAT 20 Rabbit experimental subarachnoid hemorrhage in the ultrastructure of blood vessels and pathological features and dynamic changes of Author: Song Jin-Ning, Liang Qi, Zhang, Chen Wuling [Abstract] Objective To investigate the subarachnoid hemorrhage (SAH) cerebral vascular ultrastructural features and dynamic changes, and that such change in the delayed cerebral vasospasm (CVS) in the mechanism of action. Methods of Japanese big-ear white rabbits using the second injection of blood cisterna magna SAH model of the rule of law to do. Animals were randomly divided into SAH group, saline control group, puncture the control group and normal group, in the blood injection after 1h, the first 3 days, the first 5 days, 7 days, the first 10 days of perfusion fixation, specimens from the basilar artery specimens, in the light microscopy and electron microscopy of dynamic observation of basilar artery pathology and ultrastructure changes. SAH model group, the results of light microscope, the main manifestations of vascular wall thickening, stenosis, endothelial cell degeneration, swelling, uneven chromatin, vacuolization; tortuous folds of internal elastic membrane or rupture. Electron microscopy the ultrastructure of the basilar artery is mainly tight junctions between endothelial cells disappear, the membrane partially or completely detached cytoplasm, swelling of mitochondria cristae disorder, was dissolved in vacuoles, dense granules increased nuclear chromatin margination, condensed heterochromatin increased; smooth muscle cell deformation, nuclear distortion, uneven chromatin, muscle disorder loose wire arrangement, there broken or dissolved, the formation of cytoplasmic vacuoles seen in a large number of mitochondria increased, swelling, cristae disorder or dissolved; vascular adventitial nerve fiber swelling, structural ambiguity. Light microscope basilar artery structural changes in trends and electron microscopy of structural changes in ba