Rabbit large-conductance calcium-sensitive potassium channel β subunits Cloning and bioinformatics analysis.doc

 PAGE \* MERGEFORMAT 12 Rabbit large-conductance calcium-sensitive potassium channel β subunits Cloning and bioinformatics analysis Authors: ZHANG Xiao-yong, Cuiguang Bin, Du Pang, Mark Jun, Wang Yarong, Han Wei, Yan Zhen, ZHANG Ying-Qi, Wei Jing-Guo [Abstract] Objective: To clone New Zealand rabbits were large-conductance calcium-sensitive potassium ion channel (BKCa) β subunit gene, to observe the tissue distribution, and its bio-information analysis. Methods: 3 ‘and 5’cDNA end of the fast - amplification (RACE) and RT  PCR technology from the rabbit Oddi sphincter (SO) organization to BKCa channel β subunits, the full-length cDNA sequences. and application of bioinformatics analysis of the gene homology, protein domain and the transmembrane topology. Results: The rabbit BKCa channel β subunit gene cDNA full-length 1168 bp, open reading window length of 576 bp, encoding 191 amino acids. bioinformatics analysis indicates that the gene nucleic acid sequences and amino acid sequences with human and other mammals have a high homology; topological structure of two transmembrane protein with four functional domains, containing a phosphorylation site and two N  glycosylation sites. Conclusion: The first successful from the rabbit SO organization cloned BKCa channel β subunit genes, to gain new GenBank No. DQ821756, for further study the function of the gene basis. [Keywords:] calcium-sensitive potassium ion channel 0 Introduction Large-conductance calcium-sensitive potassium ion channels (large conductance Ca2 sensitive K channels, BKCa) is widely distributed in smooth muscle cells (SMC), whose main physiological role is to regulate SMC membrane potential and muscle tension [1]. BKCa channel is composed of α -subunit and β subunits together form, where α subunit is the structural subunit, constitute pore, expression is relatively constant; β subunit is a regulatory subunit, can increase the sensitivity of calcium ion channels, the level of expression