Rac1 in human hepatoma cell lines and the significance of invasion.doc

 PAGE \* MERGEFORMAT 15 Rac1 in human hepatoma cell lines and the significance of invasion [Abstract] Objective: To confirm that Rac1 in liver cancer cell growth and invasion in the sense, looking for possible new target for cancer gene therapy. Methods: The specific siRNA inhibited the expression of Rac1 protein in highly metastatic hepatocellular carcinoma cell line interference 97 H, observed highly metastatic hepatocellular carcinoma cell lines in 97 H Rac1 expression, cell growth, viability and in vitro tumor cell invasion. Results: highly metastatic hepatocellular carcinoma cell lines in 97 H Rac1 expression, cell growth inhibition, tumor cell viability and significantly reduced invasion in vitro. Conclusion: Rac1 reduced expression can significantly inhibit the growth and invasion of hepatoma cells, may become a new target for cancer gene therapy. [Keywords:] Rac1 siRNA cell lines with high metastatic liver cancer move [Abstract] Objective: To prove Rac1 might play an important role in the proliferation and transference of hepatoma cell.Methods: small interference RNA technique was employed to knock down gene expression of Rac1 in hepatoma cell line 97 H. Results: The protein level of Rac1, the cell proliferation, the cell vitality and the ability to invade the reconstituted basement membrane were decreased dramatically after transfection with a Rac1 specific siRNA vector. Conclusion: Rac1 might play an important role in the development, progression, transference of hepatoma and also provide a possible strategy for the interference of tumor angiogenesis by targeting the Rac1. [Keywords:] Rac1; hepatoma cell; siRNA; transference Gene therapy of liver cancer are continuing to explore, the expression and regulation of treatment is an important strategy for gene therapy. In a previous study, we found that Rac1 expression in HCC cell lines was significantly higher than normal liver cells, suggesting that Rac1 and liver cancer growth and metasta