Rapid aging of learning and memory defects in mice and the biochemical mechanisms.doc

 PAGE \* MERGEFORMAT 23 Rapid aging of learning and memory defects in mice and the biochemical mechanisms [Abstract] Objective of senescence accelerated mouse (SAMP8) simulation of Alzheimer’s, to observe the learning and memory ability and learning and memory ability of the possible mechanisms. Methods 10 months old SAMP8, and with the age of senescence accelerated mouse resistant (SAMR1) as normal controls. water lost memory and spatial learning test in recent memory. detected by biochemical mitochondrial membrane potential and ATP cortical activity observed mitochondrial function, detection cortex and hippocampus of choline acetyltransferase (ChAT) and acetylcholine ester enzyme (AChE) of the activity, observe the central cholinergic function, serum superoxide dismutase (SOD) activity and malonaldehyde (MDA), to observe the systemic oxidative stress conditions. Results Compared with SAMR1, SAMP8 obvious aging signs, which in recent memory and spatial learning and memory significantly decreased with aging and dementia characteristic cortical mitochondrial membrane potential and the ATP enzyme activity was significantly reduced hippocampal ChAT activity was significantly decreased, AChE activity was significantly increased serum SOD activity slightly higher. Conclusion SAMP8 can simulate dementia, the mechanism of reduced mitochondrial function, including cerebral cortex, hippocampus and cholinergic nerve function decreased oxidative stress. [Keywords:] Alzheimer senescence accelerated mouse mitochondrial oxidative stress in cholinergic ABSTRACT: Objective To simulate senile dementia with senescence accelerated mouse / prone 8 (SAMP8) and investigate its learning and memory deficits, and their biochemical mechanisms. Methods The general status of SAMP8 was investigated compared with that of SAM / resistance 1 (SAMR1). Then their recent memory and spatial learning and memory abilities were detected with water maze. The mitochondrial membrane potential