Quality Standard of Hong Yuan.doc

 PAGE \* MERGEFORMAT 26 Quality Standard of Hong Yuan [Abstract] Objective To establish quality standards for particles Kang Yuan. Methods TLC prescription astragalus, peony root, wolfberry fruit and hay were identified by high performance liquid chromatography method for the determination of paeoniflorin in preparation, the use of AichromBond- AQ-C18 column (4.6 mm × 150 mm, 5 μm), mobile phase: water - acetonitrile (85:15), detection wavelength was 230 nm, column temperature 30 ℃, the flow rate 1 mL / min. the results of post-TLC high specificity, linear range of paeoniflorin 0.0545 ~ 0.545 μg, r = 0.999 5, the average recovery was 97.90%, RSD = 1.06% (n = 6). Conclusion The method is simple, accurate and reproducible , Yuan Kang particles can be used as quality control standards. [Keywords:] Hong Yuan particles, paeoniflorin, high performance liquid chromatography, thin layer chromatography; quality standards Abstract: Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond- AQ-C18 (4.6 mm × 150 mm, 5 μm) was used, the mobile phase was Water-Acetonitrile (85:15), at the flow of 1.0 mL / min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545 ~ 0.545 μg, r = 0.999 5, the average recovery was 97.90%, RSD = 1.06% (n = 6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation. Keywords:: Kangyuan Granula, Paeoniflorin, HPLC, TLC, quality standard Kang Yuan particles are of traditional Chinese medicine hospital 6 new class of drugs developed by the astragalus, peony root, an