Rat dental follicle cells culture method of.docVIP

 PAGE \* MERGEFORMAT 4 Rat dental follicle cells culture method of [Abstract] [Objective] To investigate the rat dental follicle cells in vitro methods. [Method] isolated rat dental follicle, namely the use of tissue culture method, cell digestion dispersion method, and modified tissue explant for dental follicle cells in primary and passaged culture, carried out under the inverted phase contrast microscope, cell morphology, transmission electron microscopy observation of cell ultrastructure. [Results] culture to 3 days, inverted phase contrast microscope observations, using tissue culture method, see a small number of cells from tissue climbing; while the number of cells available to digest and more decentralized rule dental follicle cells, but mixed with the impurities in the growth of cells, ; modified tissue explant see the quantity, relatively pure dental follicle cells. Electron microscope, no desmosome dental follicle cells, the cytoplasm contains a high density of electronic particles and large amounts of rough endoplasmic reticulum. Explant, cell dispersion method and improved digestion method dental follicle cells in tissue culture success rate was 80%, 60% and 100%; respectively, 10 d, 7 d and 6 d or about the first passage. [Conclusion] The modified tissue explant is a good rat dental follicle cells culture method. [Keywords:] dental follicle cells; cell culture; methodology Abstract: [Objective] To explore the culture method of rat dental follicle cells in vitro. [Methods] The dental follicle (DF) tissues were dissected for primary generation and passage culture by methods of explant, dissociation cell and improved explant, respectively. Consequently , the morphology of the cultured first passage DF cells and their ultrastructure were discovered under inversion phase difference microscope and transmission electronic microscope, respectively. [Results] At the third day in vitro, only a few cells extended from the explant by way of explant, and ma