Rb gene product in the process of rat hepatocarcinogenesis and significance of abnormal expression of.docVIP

 PAGE \* MERGEFORMAT 13 Rb gene product in the process of rat hepatocarcinogenesis and significance of abnormal expression of Of: Hanke Jiang, Wu Yali, Liangping △, Li Jing, Wang Xi-wen, Huang Xiaobing [Abstract] Objective To study the process of malignant transformation in the Rb gene product (Rb protein) expression and their significance. Methods 0.2% diethylnitrosamine (DEN) induced liver cancer model established Wistar rats, immunohistochemistry Detection of Rb protein in rat liver carcinogenesis and metastasis of carcinoma. results in the control group were seen in 20 Rb protein expression in patients with liver cirrhosis 21 18 Rb protein expression, liver cancer group (68 without metastasis There are only 37 Rb protein expression, liver cancer (18 with metastasis in 3 Rb protein expression. Conclusion The positive rate of Rb protein with the severity of lesions decreased, which secondary evidence of the Rb protein in the formation of hepatocellular carcinoma tumor suppressor role to play, Rb protein inactivation may promote the formation of liver cancer and liver cancer development and metastasis. [Keywords:] Rb gene groups of hepatocellular carcinoma in rats Abstract Objective To discuss the abnormal expression of retinoblastoma protein (Rb) gene product in the hepatocellular carcinogenesis and its significance.Methods 0.2% N-nitrosodethylamine (DEN) was applied to induce Wistar rats to form the model of hepatocellular carcinoma (HCC), and the expression of Rb protein in HCC carcinogenesis and metastasis was detected with immunohistochemical method.Results 20 rats in control group all showed the expression of Rb protein, 18 rats out of the 21 rats in liver cirrhosis group had the expression of Rb protein as well as 37 rats out of the 68 rats in HCC (without metastasis) group, and only 3 rats out of the 18 rats in HCC (with metastasis) had the expression of Rb protein.Conclusions Uhe positive expression rate of Rb protein decreases gradually w