Real-time fluorescent RT.docVIP

 PAGE \* MERGEFORMAT 12 Real-time fluorescent RT Authors: Zhen-Yong Li, Shao big Xiao, Feng Yan-ming, Li Zhitao, Jing Jian-zhou, Wang Yunlong, QU Ling-Bo, ZHAO Yu-Fen [Keywords:], SARS virus, Realtime fluorescent RTPCR quantitative detection of SARS CoV in serum of infected patients [Abstract] AIM: To detect SARSCoV in various clinical specimens such as sputum, gargling liquid, throat swab and stool and explore the association between the disease course and the SARSCoV content. METHODS: We quantitatively detected 156 serum specimens from SARS patients by real time RTPCR. The viral load in SARSCoV positive serum was analyzed using the coronavirus deactivated with 9.4 * 109 copies / L. Robert Koch Institute as standard preparation and viruslike particles as positive controls. RESULTS: The sensitivity of the detected agent was 1 * 105 copies / L. Eightyone of the 156 cases (51.9%) tested were positive with the viral load ranging from 1 * 105-1 * 107 copies / L. The viral load in serum samples was generally low as compared with data reported for other specimens. CONCLUSION : There is no significant relationship between the low viral load and the disease course. [Keywords] RTPCR; SARS CoV; quantitative diagnosis; serum [Abstract] Objective: To elaborate course of SARS in patients with serum content of the relationship between the SARS virus. Methods: The high-sensitivity fluorescent real-time quantitative RTPCR method, 156 patients with SARS were detected at the same time, to 9.4 * 109 copies / L (Robert Koch Institute)-inactivated SARS coronavirus as the quantitative standard, virus-like particles as a positive control, analysis SARSCoV positive serum samples of virus concentration. Results: The sensitivity of detection reagents up to 1 * 105 copise / L, and related pathogens do not cross. 81 serum samples of the virus concentration found in 1 * 105 ~ 1 * 107 copies / L among patients with low levels of virus in serum samples by statistical analysis, the viru