Real orange peel extract the determination of hesperidin and scavenging of free radicals.docVIP

 PAGE \* MERGEFORMAT 15 Real orange peel extract the determination of hesperidin and scavenging of free radicals Of: Chen Jianqiu, Hu Zhijun, Wang Nan Qi, Shen Wei Yang [Abstract] Objective Optimization Determination of Hesperidin in orange real experimental methods and classes in the flavanone hesperidin extraction. Methods Methods: L9 (34) orthogonal experiment, the type of solvent, color time, temperature and reagent consumption and other factors, extraction technology: use of L9 (34) orthogonal experiment to investigate ethanol concentration, solvent, extraction time and temperature factors, Fenton reaction principle with reference to implementation of different flavonoids orange the role of scavenging OH. Results Determination of Hesperidin in optimal conditions: hesperidin and 5% Al (NO3) 3 solution (m: V, reaction at 20 for 20 min, the maximum absorption wavelength 400 nm, hesperidin extract the optimum conditions: 70% ethanol as extraction solvent, solid to liquid ratio of 1:20 (m: V, at 50 for 3 h. Extraction of medium speed mixing real orange extract flavonoids OH generated by Fenton system has some scavenging. Conclusion The optimized extraction using different extraction of hesperidin in orange real, found the highest content of hesperidin in orange peel. [Keywords:] hesperidin, extraction process, orthogonal, determination, free radical scavenging Abstract: ObjectiveTo study the determination method of hesperidin content by spectrophotometer and extraction technology of hesperidin from peels of citrus.MethodsAnalysis technology: The type of solvent, temperature, quantity of indicator and the time of reaction were investigated through orthogonal tests of L9 (34) , Extraction technology: The concentration of ethanol, quantity of ethanol, time and temperature of extraction were investigated through orthogonal tests of L9 (34). In addition, the scavenging effect of flavanone from peels of citrus on hydroxyl radical was studied.ResultsThe