Real-time quantitative reverse transcription polymerase chain reaction detection of colorectal cancer patients with CK20 mRNA in peripheral blood before surgery and clinical significance of CEA mRNA.docVIP

 PAGE \* MERGEFORMAT 13 Real-time quantitative reverse transcription polymerase chain reaction detection of colorectal cancer patients with CK20 mRNA in peripheral blood before surgery and clinical significance of CEA mRNA Of: Zhou Hai Yan, Gecheng Hua, Wang Shiwei, leaves the ancient Xiang, Tang Wenxian, Zhaoxu Hong, Jenny Fan [Abstract] Objective To investigate the peripheral blood of patients with colorectal CEA mRNA and CK20 mRNA expression and clinical significance. Methods Using real-time quantitative reverse transcription polymerase chain reaction (QRT-PCR technique in 68 patients with colorectal cancer and 30 healthy volunteers (control group preoperative CK20 mRNA and CEA mRNA in peripheral blood were detected. Results In the control group had no CEA mRNA and CK20 mRNA expression in colorectal cancer group, positive expression of CK20 mRNA was 45.6%, CEA mRNA expression rate 39.7%, both positive detection rate was 55.9% combined compared to individual testing positive rate (Pgt; 0.05, both expression and sex, tumor location and tumor differentiation had no correlation with clinical stage, lymph node metastasis and liver metastasis was significantly related. Conclusion CK20 mRNA and CEA mRNA blood of colorectal cancer may be disseminated as a symbol of the joint detection helps to improve the detection rate of tumor micrometastasis and prognosis. [Keywords:] colorectal cancer micrometastases in peripheral blood [Abstract] Objective To investigate the expression of CK20 mRNA and CEA mRNA in peripheral blood of patients with colorectal cancer and its clinical significance.Methods CK20 mRNA and CEA mRNA were detected in 68 patients with colorectal cancer before operation, 30 health adults by QRT- PCR.Results CK20 mRNA and CEA mRNA were undetected in the control group, the positive rate of CK20 mRNA among colorectal cancer patients was 45.6%, and of CEA mRNA was 39.7%. The positive rate of combination detection was 55.9%. It was higher than the