Real-time quantitative PCR bone marrow mesenchymal stem cells transplanted into infarcted myocardium survived after the number of.docVIP

 PAGE \* MERGEFORMAT 12 Real-time quantitative PCR bone marrow mesenchymal stem cells transplanted into infarcted myocardium survived after the number of Of: Wang Yeh Huan, Zhang Hao, Yuan Xin, Hou Jianfeng, Liu Xuebin, Ye Jue, Sun Cheng Chao [Abstract] Objective: To establish a stem cell transplant to test their survival after myocardial infarction in the number of effective methods. Methods: 10 female SD rats with ligation of left anterior descending artery, myocardial infarction 3 weeks after transplantation were randomly divided into two groups, select both male rats cultured bone marrow mesenchymal stem cells (MSCs), respectively MSCs (3 106 , 50L) and an equal volume of PBS solution transplanted into infarcted myocardium, 24 h after the real-time quantitative PCR (Real-time PCR), male rats on the Y chromosome-specific gene SRY, so that the number of transplanted MSCs to survive. Results: MSCs 24 h after transplantation, transplantation of myocardial infarction detected in the total number of SRY gene transfer accounted for 14.9% + -8.3 %, the absolute number of surviving cells 447 195 + -248 090, while the control group were not detected the presence of SRY gene. Conclusion: Real-time PCR method can detect the SRY gene in a stem cell, which can accurately to learn that the number of transplanted stem cells survive, Real-time PCR technology in stem cell transplantation after myocardial infarction and the clinical application of basic research has important value. [Keywords:] real-time quantitative PCR; myocardial infarction; cell transplantation Abstract: Objective: To establish an effective method to detect surviving number of the stem cells in vivo. Methods: Ten adult female SD rats were randomly assigned into two groups: the bone marrow derived from mesenchymal stem cells (MSCs) transplantion group and the control group. Anterior myocardial infarction was induced by ligation of the proximal left anterior descending artery. Using an insulin