RECK in human laryngeal squamous cell carcinoma and its clinical significance.doc

 PAGE \* MERGEFORMAT 14 RECK in human laryngeal squamous cell carcinoma and its clinical significance [Abstract] Objective To detect the 80 cases of human laryngeal squamous cell carcinoma of the expression of RECK, discuss the development of RECK in laryngeal squamous cell carcinoma in the role. Methods Using immunohistochemistry, Western blot analysis (Western blot) technique, reverse transcriptase polymerase chain reaction (RT-PCR) detection of laryngeal squamous cell carcinoma tissue specimens in the expression of RECK. Results RECK protein in normal laryngeal tissue and laryngeal squamous cell carcinoma of the large cancer nests was not expressed, and only in the mucous membranes in the keratin pearls lamina propria in the glandular cells mixed gland expression. Conclusion This study suggests the occurrence of RECK gene in the development of laryngeal not critical. [Keywords:] laryngeal squamous cell carcinoma; RECK gene Abstract: Objective To discuss the potential role of RECK in Laryngeal squamous cell carcinoma (LSCC) by testing the expression of RECK in human LSCC, a total of 80 LSCC patients. Methods (1) RECK expression in surgically resected tissue samples of laryngeal carcinomas ( n = 80) were examined immunohistochemically; (2) The expressions of RECK was evaluated by Western blot and Reverse transcription polymerase chain reactions (RT-PCR). Results The positive RECK stained in the horny pearl and glandular tube cells, but not in the carcinomal cells. Conclusions RECK gene can t be considered as a key point in the pathogenesis of LSCC. Keywords:: Laryngeal squamous cell carcinoma (LSCC); RECK gene Carcinoma of head and neck second primary in the epithelium of the most common malignant tumor, the incidence rate after the NPC. In the past 40 years on the pathogenesis of laryngeal cancer has made much progress, but the occurrence of laryngeal The exact mechanism of the molecular mechanisms of invasion and metastasis is not very clear.