Reconstruction of an asthma attack and the cytoskeleton-related genes differentially expressed in.docVIP

 PAGE \* MERGEFORMAT 14 Reconstruction of an asthma attack and the cytoskeleton-related genes differentially expressed in Author: Cai Shao-xi, Zhao Jin, Yan-Qing Ding, Wenjun Li, ZHOU Bin, XIAO Jun [Keywords:] Suppression subtractive hybridization; asthma; eosinophils; Cytoskeletal proteins Differentially expression of genes associated with cytoskeletal remodeling of peripheral blood eosinophils during asthmatic attack [Abstract] AIM: To determine the differentially expressed genes associated with cytoskeletal remodeling of peripheral blood eosinophils (EOS) in patients during asthmatic attack. METHODS: Doublestranded cDNA was amplified from total RNA of EOS at the time of asthmatic attack and after improvement respectively by Super SMART cDNA Synthesis techology, Suppression subtractive hybridization (SSH) and PCR select differential screening technology were used to detect differentially expressed genes and then differentially expressed genes were sequenced. Homology analysis was conducted by comparing these gene sequences based on GenBank database. RESULTS: Highefficiently upregulated and downregulated subtractive cDNA libraries were constructed successfully; differential screening and homology analysis identified 6 differentially expressed genes associated with cytoskeletal remodeling, including slingshot 2L (SSH2L), PP1 catalytic subunit, beta isoform (PP1Cβ ), dedicator of cytokinesis 8 (DOCK8), Homo sapiens protein tyrosine phosphatase nonreceptor type 6 and 12 (PTPN6, PTPN12), myosin regulatory light chain interacting protein (MIR). CONCLUSION: The differentially expressed genes associated with cytoskeletal remodeling may participate in exacerbation of asthma. Further study on the genes function may provide new gene target for asthma treatment. [Keywords] suppression subtractive hybridization; asthma; eosinophils; cytoskeletal proteins [Abstract] Objective: To study the peripheral blood eosinophils (EOS) during an asthma attack associated with t