Rat aortic allograft chronic disease model.docVIP

 PAGE \* MERGEFORMAT 4 Rat aortic allograft chronic disease model [Keywords:] abdominal aortic graft with chronic cardiac allograft vasculopathy of chronic rejection Chronic rejection has become an obstacle to graft an important reason for long-term survival, chronic rejection is the main pathological features of chronic graft vascular disease. Immunity and the role of non-immune factors leading to chronic cardiac allograft vasculopathy [1,2], so far no effective drug used clinically to enhance basic research in this area has become increasingly important. Through the establishment of simple, practical animal model of chronic better able to carry out basic research in cardiac allograft vasculopathy. I rat aortic model of chronic graft vascular disease, the report is as follows. 1 Materials and methods 1.1 Experimental animals and groups were randomly closed to the use of cleaner-class healthy male inbred Brown-Norway rats and inbred Lewis rats. Inbred Brown-Norway donor rats, weighing 200 ~ 350 g, inbred Lewis rats were used as recipients, weighing 200 ~ 350 g. All rats were purchased from Beijing Tong Lihua-dimensional experimental animals Technology Co., Ltd.. Experiment is divided into three groups: control group A for the Lewis rats were sham-operated group; the control group B is between Lewis ~ Lewis rats transplanted with the Department of abdominal aorta; experimental group C for the Brown-Norway to Lewis rats with allogeneic abdominal aorta transplantation. Three-component after 3,7,15,30,45,60 d 6 hour-time-phase points, for each time point 8 each right. 1.2 in rat aortic transplantation model in preparation for, the receptor preoperative therefore correspondingly 1% pentobarbital sodium 40 mg / kg intraperitoneal injection of anesthesia, clipping for the Brown-Nor-way rat abdominal aorta 2 ~ 3 cm, orthotopic transplantation into the recipient mice Lewis abdominal aorta. Surgery total time 50 ~ 60 min, vascular anastomosis a total of about 16