Rat liver ischemia-reperfusion on bile duct fiber-shaped filaments of actin.docVIP

 PAGE \* MERGEFORMAT 16 Rat liver ischemia-reperfusion on bile duct fiber-shaped filaments of actin Of: Li Yiming, Liu Jidong, Ji Hong, Shun-Le [Abstract] Objective To investigate the hepatic ischemia-reperfusion (I / R) of the bile duct-shaped actin fibers (F actin) filaments. Methods used model of hepatic ischemia and 35min reperfusion, serum ALT, AST , GGT, and TBIL content of bile duct changes in electron microscopy, hydrogen guanidine isothiocyanate fluorescein labeled phalloidin (FITC Phalloidin) showed bile duct changes in the distribution of F actin filaments, confocal laser scanning microscopy and quantitative analysis of images collected . Results F actin filaments fluorescence changes and ultrastructural changes consistent with electron microscopy, F actin fluorescence staining normal before reperfusion, and significantly decreased after reperfusion, bile canalicular microvilli did not disappear before reperfusion, and reperfusion was large loss. This is the abnormal serum GGT and TBIL change. Conclusion reperfusion rather than ischemia can cause damage to bile duct F actin filaments, loss of microvilli, resulting in reduced contraction of bile duct, bile excretory function impairment. This may be hepatic I / R occurred after the main mechanism of cholestasis. [Keywords:] liver ischemia, cholestasis, fiber-shaped F actin ABSTRACT: Objective To investigate the effect of hepatic ischemia reperfusion (I / R) on bile canalicular F actin microfilaments in rat. Methods A model of rat hepatic ischemia reperfusion was adopted and the ischemia time was 35min. The levels of serum ALT, AST, GGT, and TBIL were measured. The changes of bile canaliculus were observed by transmission electron microscope. The modification of F actin microfilaments was quantified by using FITC Phalloidin and analyzed by confocal laser scanning microscopy imaging. Results This modification of F actin staining was consistent with the observation by transmission electron mic