Rat islet isolation and purification of cell loss.docVIP

 PAGE \* MERGEFORMAT 13 Rat islet isolation and purification of cell loss [Abstract] Objective To improve the yield and purity of islet cells the best separation and purification methods. Methods 40 rats were divided into 4 groups of 10 each. A Group: Hanks collagenase perfusion + digest + Ficoll400 gradient purified V , B Group: Organization cut and ground + mesh filter, C Group: V collagenase perfusion + Ficoll400 gradient purified, D group: perfused with collagenase V mesh filter. of purified using four different methods to compare the results. The results fully randomized design analysis of variance show, A, B, C, D and purity harvest group had significant difference (P lt;0.05), multiple comparisons between groups show, A group of harvest volume than B, D group (P lt;0.05), compared with C group no significant difference (Pgt; 0.05), A group of purity and other groups had no significant difference (Pgt; 0.05). Conclusion V or Hanks collagenase perfusion, Ficoll400 gradient purification of the islet cells harvested yield and high purity and low price, is an ideal method for isolation and purification of pancreatic islet cells. [Keywords:] Purification of rat pancreatic islet cells ABSTRACT: Objective To explore the best method of isolating and purifying rat islet cells to improve the harvest and purity of rat islet cells. Methods Forty rats were divided into four groups, with ten rats per group. Group A: Rat pancreases were perfused with Hanks and digested by collagens V, islet cells were isolated and purified by gradient Ficoll400. Group B: Pancreases were cut and ground, then filtered with grid. Group C: Rat Pancreases were perfused with collagens V, islet cells were isolated and purified by gradient Ficoll400 . Group D: Rat pancreases were perfused with collagens V, but filtered by grid. The harvest and purity of islet cells from four groups were compared and analysed. Results The differences in the harvest and purity of islet cells isolated and