Rat model of chronic aristolochic acid nephropathy in renal tissue differences in expression proteomics.docVIP

 PAGE \* MERGEFORMAT 5 Rat model of chronic aristolochic acid nephropathy in renal tissue differences in expression proteomics Author: Sun Shiren, Guang-Lei Chen, Ning Xiao Xuan, Wei [Keywords:] aristolochic acid; rats; kidney tissue; two-dimensional polyacrylamide gel electrophoresis; proteome Differentially expressed proteins in renal tissues of rat models of chronic aristolochic acid nephropathy [Abstract] AIM: To establish the 2dimensional polyacrylamide gel electrophoresis (2D PAGE) profiles from aristolochic acid nephropathy (AAN) rat renal tissues and normal renal tissues and to identify the differentially expressed proteins. METHODS: Establish chronic renal interstitial fibrosis model in rats by peritoneal injection with aristolochic acid (AA). The total proteins from AAN models and normal rat renal tissues were separated by 2D PAGE. The differentially expressed proteins in 2DPAGE maps were analyzed using image analysis software. RESULTS: The rat models of AAinduced chronic renal interstitial fibrosis were established successfully. Clear, wellresolved and reproducible 2D PAGE patterns of AAN models and normal rat renal tissues were obtained and many differentially expressed proteins were found. CONCLUSION: The differentially expressed proteins of renal tissues can be induced by AA in Wistar rats. These proteins maybe mediate the toxicity of aristolochic acid to renal tissues. [Keywords] aristolochic acid; rats; renal tissues; 2D PAGE; proteome [Abstract] Objective: Preparation of chronic aristolochic acid nephropathy rat model, the establishment of aristolochic acid nephropathy and normal renal tissue of rats with two-dimensional electrophoresis map, which show differential expression of proteins for further screening mediated toxic effects of aristolochic acid in the protein molecule basis. Methods: Preparation of aristolochic acid in rats by intraperitoneal injection model of chronic aristolochic acid nephropathy, extracting the total protein