Rat muscle-derived stem cells in primary culture and identification.docVIP

 PAGE \* MERGEFORMAT 12 Rat muscle-derived stem cells in primary culture and identification [Abstract] Objective To investigate the skeletal muscle-derived stem cells in vitro, identification methods. Methods I two-step collagenase digestion and trypsin isolated rat skeletal muscle-derived stem cells were purified by differential adherence, the culture medium in culture. inverted microscope cell morphology, growth curve analysis muscle-derived stem cell proliferation, desmin immunofluorescence identified muscle-derived stem cells. Results After two steps of digestion and differential adhesion, the successful cultivation of high purity muscle-derived stem cells, immunofluorescence, the results of desmin (+). conclusions obtained by in vitro primary culture of highly purified muscle-derived stem cells, desmin immunofluorescence for its early identification, clinical application of tissue engineering to provide seed cells. [Keywords:] rat muscle-derived stem cells in vitro Abstract: Objective To establish the methods for culture and identification of rat muscle-derived stem cells in vitro.Methods By two-step method, the rat muscle was digested with type I collagen and trypsin, and the isolated cell suspension was purified by different adhesion time. The proliferation ability of muscle-derived stem cells was analysed through studying growth curve, and the obtained cells were identified with cell immunofluroescence technique. Results Highly purified MDSCs were harvested and they showed desmin (+) by cell immunofluroescence technique. Conclusions MDSCs can be cultured in vitro and can be identified by desmin stain and used to tissue engineering as “seed” cells. Keywords:: rat; muscle derived stem cells; culture in vitro Recent studies have found that adult skeletal muscle, not only there is a single energy into the muscle stem cells - satellite cells, but also contains more energy, “muscle-derived stem cells” (muscle derived stem cells, MDSCs. Muscle