Rate of pyruvate oxidase method in the detection of large quantities of blood donors in the application of ALT.docVIP

 PAGE \* MERGEFORMAT 6 Rate of pyruvate oxidase method in the detection of large quantities of blood donors in the application of ALT [Abstract] Objective: pyruvate oxidase rate method for large quantities of ALT testing of blood donors. Methods: Oxidation of pyruvate by pyruvate oxidase generates hydrogen peroxide, hydrogen peroxide and 2,4,6-tribromo -3-- hydroxy acid (TBHBA and 4 - amino ammonia antipyrine (4-AAP under the action of the peroxidase to form a red quinone. Results: The linear range up to 200 U / L, and the enzyme-coupled UV-rate method comparison, the correlation coefficient r = 0.991, y = 1.072x +1.032, n = 90. Conclusion: This method is simple, rapid, accurate, sensitive and reproducible, especially for large quantities of blood donors Blood ALT test. [Keywords:] blood donors, ALT, pyruvate oxidase rate method At present, the levels of blood centers, blood banks commonly used alanine aminotransferase (ALT) referred to as the detection method depends on ALT’s method, rate method, ketone body powder method, but Lai’s method (60 min) reaction time is longer, ketone body powder method the accuracy is poor, require a higher rate method of instrument characteristics, are not suitable for large-scale blood banks blood donation grassroots activities [1]. I stood since 2005, with the rate of pyruvate oxidase method, the method uses microplate reader to the grassroots blood bank necessary, but simple, fast and with high accuracy and precision, ideal for grass-roots donors ALT Blood volume control. 1 Materials and methods 1.1 Instrument Microplate reader (Multiskan MK3, Labsystems, Finland company, TDA40FA automatic biochemical analyzer (Toshiba. 1.2 Reagents ALT / GPT kit (Jiangxi Medical preparations Ruihua Biochem Co., Ltd., batch number20060205. 1.3 Quality Control Serum Ruihua Jiangxi Biochem Co., Ltd. provides medical preparations, confirmed by automatic biochemical analyzer, low serum (L): 51 U / L, high