Rat reflux esophagitis and Barrett esophagus Comparison of gene expression profiles.docVIP

 PAGE \* MERGEFORMAT 3 Rat reflux esophagitis and Barrett esophagus Comparison of gene expression profiles Author: Peng, Gong Jun, Gui-Sheng Liu, Wang Tao, Chang-ying [Abstract] Objective: To study the Barrett esophagus (BE) and reflux esophagitis (RE) gene expression profile different. Methods: Mixed gastroduodenal reflux SD rat animal model, using contains 4096 pairs of point in rats and compared cDNA chip BE, RE and normal esophageal epithelium (NC) mRNA expression. Results: The microarray hybridization of differentially expressed genes in the three times, RE, and NC-hybrid chip 232, in which increased expression of genes 90, 142 genes were down-regulated; BE, and NC-hybrid chip 448, in which increased expression of 312 genes, 136 genes were down-regulated; relative to the RE, BE increased expression of 214 genes, 86 genes were down-regulated. Conclusions : BE and RE at the level of gene expression differences. [Keywords:] Barrett Esophagus 0 Introduction In recent years, esophageal adenocarcinoma (esophageal adenocarcinoma, EA) incidence rates showed a rising trend in the world, Barrett esophagus is considered a major risk factor for its occurrence cause for concern. But its mechanism is unclear. We have applied Miwa, etc. (Int J Cancer, 1996, 67:260) Methods mixed gastroduodenal reflux animal models, gene-chip technology, dynamic observation of esophageal epithelium and excessive exposure to gastric and duodenal reflux material mixed in the expression of gene transcription changes, with a view to clear BE occurrence and development of molecular biological mechanism for the EA. 1 Materials and methods 1.1 Material 2 SD rats were 120 experimental animals, 8 wk age, body weight (220 ± 20) g, were purchased in Experimental Animal Center of Xi’an Jiaotong University, SPF male and female sub-cage rearing conditions. Male and female rats were paired randomly divided into experimental group 90, control group 30, each male and female evenly divided. using m