Rat trigeminal ganglia in vitro can be used for vascular headache pathogenesis and drug therapy target of.docVIP

 PAGE \* MERGEFORMAT 19 Rat trigeminal ganglia in vitro can be used for vascular headache pathogenesis and drug therapy target of Of: Luoguo Gang, Nelly, Lu Shemin, Fan Wenjing, Xu Bao positions [Abstract] Objective carotid artery elevated levels of calcitonin gene-related peptide is a cause of vascular headache is one of the important reasons, this study was to investigate the occurrence of vascular headache mechanisms and drug targets for the ideal in vitro model. Methods large SD rat trigeminal ganglia in serum-free DMEM medium 12,24,48 h after in vitro comparison of immunohistochemistry of calcitonin gene-related peptide (CGRP) expression of positive cells, real-time quantitative PCR method (real time PCR) to determine CGRP mRNA expression levels. Results rat trigeminal ganglion after 24h in vitro expression of CGRP immunopositive cells significantly increased, CGRP mRNA expression levels were significantly higher than that of fresh (P all lt;0.05). TRIGEMINAL section after in vitro able to accurately simulate the in vivo vascular changes in migraine attacks of CGRP is to study the mechanism of vascular headache and development of drug targets for the ideal in vitro model. [Keywords:] trigeminal ganglion, in vitro culture, calcitonin gene-related peptide, in vitro model ABSTRACT: Objective To explore an ideal model for primary vascular headache in studying pathogenesis and new medication therapeutic target in vitro. Methods Trigeminal ganglion (TG) of SD rats was isolated and cultured in serum free DMEM medium for 12h, 24h and 48h. Then the calcitonin gene related peptide (CGRP) positive stained cells were detected by using immunohistochemistry staining, and real time polymerase chain reaction (RT PCR) was used to determine mRNA expression changes of CGRP. Results The TG cultured in serum free DMEM for 24h had a significant increase in the CGRP positive stained cell number and upregulated strikingly CGRP mRNA expression compared with fresh TG (P