Ratio of different doses of an experimental hepatitis A hepatitis combination vaccine immunogenicity study of.docVIP

 PAGE \* MERGEFORMAT 9 Ratio of different doses of an experimental hepatitis A hepatitis combination vaccine immunogenicity study of [Keywords:] hepatitis A virus;,, hepatitis E virus;,, joint vaccines;,, dose ratio;,, immunogenicity Immunogenicity of experimentally combined hepatitis A and hepatitis E vaccine at different dosage ratio [Abstract] AIM: To evaluate the immunogenicity of experimentally combined hepatitis A and hepatitis E (cHA E) vaccine and analyze the interactions between the two antigenic components in vaccine. METHODS: Nine different dosage ratio of the cHA E vaccine, A500 E200, A500 E100, A500 E50, A250 E200, A250 E100, A250 E50, A125 E200, A125 E100 and A125 E50 (U mg) / L, was prepared from an inactivated hepatitis A (HA) vaccine and a hepatitis E (HE) vaccine. 120 mice were immunized with the cHA E vaccine, HA vaccine and HE vaccine alone in different doses, respectively. Mice were bled before immunization and 2, 4, 6, 8, 10, 12 and 14 weeks post immunization. Specific antibodies against hepatitis A and E viruses were detected by ELISA and neutralization assay. RESULTS: After immunization with different dosages of cHA E vaccine, all animals developed hepatitis A virus antibody (antiHAV) and hepatitis E virus antibody (antiHEV). AntiHAV responses increased with the concentration of the HA vaccine component in the cHA E vaccine. AntiHAV neutralizing antibody titers reached 1:1 024 when mice were immunized with cHA E vaccine prepared from higher dosage (500 U / mL) of the HA vaccine. The titers only reached 1:512 when lower dosages (250 and 125 U / mL) of HA vaccine were applied. However, different dosages (200, 100 and 50 mg / L) of the HE vaccine component in the cHA E vaccine developed no effects on the production of antiHAV neutralizing antibody. In addition, comparing with the single HE vaccine, the cHA E vaccine induced higher level of antiHEV response. The antiHEV level increased with the concentration of HA vaccine component in the