Rat brain Qingnaoxuanqiao side Geniposide index components such as 3 by High Performance Liquid Chromatography.docVIP

 PAGE \* MERGEFORMAT 14 Rat brain Qingnaoxuanqiao side Geniposide index components such as 3 by High Performance Liquid Chromatography [Abstract] Objective To establish a rat brain Qingnaoxuanqiao been effective part in the three index components geniposide, ginsenoside Rg1, Rb1 in the HPLC concentration determination. Methods in rat brain samples removed, wash away the residual blood, absorbed pulp, extracted with methanol, water-soluble after centrifugation, with the C18 SPE column treatment to Hai Mao phenolic glycosides as the internal standard, the choice of column: Agilent Eclipse XDB-C18 (4.6 mm 250 mm, 5 m), Eclipse XDB-C18 guard column. acetonitrile - formic acid gradient elution, the column temperature was 30 , the flow rate was 1.0 ml / min, detection wavelength was 203 nm. The results of each component of the minimum detection limit (S / Ngt; 3) in the 0.16 ~ 1.1 mg / L, the average recoveries were more than 90% day precision and stability of the RSD were less than 10%. Conclusion The method is simple, accurate, and can be used to clear the brain declared brain Khieu been effective part of geniposide, ginsenoside Rg1, Rb1 3 kinds of components quantitative analysis. [Keywords:] Qingnaoxuanqiao party; Geniposide; ginsenoside Rg1; ginsenoside Rb1; high performance liquid chromatography Quantitative Determination of 3 Main Components in Effective Fraction of Qingnaoxuanqiao Formula in SD Rat Brain by RP-HPLC Abstract: ObjectiveTo establish a method for determination of the concentrations in rat brains of geniposide, ginsenoside Rg1 and ginsenoside Rb1 by HPLC in the effective fraction of Qingnaoxuanqiao Formula. MethodsThe rat brain samples were taken out, cleaned remaining blood, smashed into plasm, extracted by MeOH, and dissolved by water after centrifuging. A column of C18 solid phase was used for the extraction and Sec-o-glucosylhamaudol was taken as internal standard. The procedure was carried out on a chromatography column of Agilen