Rat pancreas during development Munc13.docVIP

 PAGE \* MERGEFORMAT 14 Rat pancreas during development Munc13 Authors: Qing-Xin, Liu Chao, Teng Liping, Liu Cui-ping, ZHOU Jin-Yong, Guo Jing, CHENG Mei, david [Keywords:] embryonic pancreas development Effect of Munc131 on insulin secretion during pancreatic development in rats [Abstract] AIM: To investigate the expression of Munc131 during various stages of embryonic pancreatic development in rats and define the effect of Munc131 on insulin secretion. METHODS: Pancreata of rat embryonic day 12.5 (E12.5), E15.5, E18. 5, newborn, 21 d after birth (P21) and adult rats were dissected under microscope respectively. In addition, the animal models of intrauterine growth retardation (IUGR) in rats were made by 50% calorie restriction in pregnant rats from gestational day 15 until term. After abstraction of totle RNA and protein, the techniques of RTPCR, realtime PCR, Western blot and ELISA were used to study the expression of Munc131 and insulin secretion. RESULTS: The genes of insulin and Munc131 began to be expressed from E12.5 and E15.5 respectively and their expressions were increased as the fetus grew. The result of Western blot showed that the expression of Munc131 was low at E15.5 and E18.5 and increased later. Blood insulin level was detected at E18.5 and increased rapidly thereafter. The blood insulin level and the expression of Munc131 were reduced simultaneously in IUGR models compared with normal newborn rats. CONCLUSION: The expression of Munc131 is accordant to blood insulin level and plays an essential role in insulin exocytosis. [Keywords] Munc131; insulin / secretion; embryonic pancreatic development; diabetes mellitus; IUGR [Abstract] Objective: To study the different stages of development of rat pancreas Munc131 gene and protein expression and insulin release in the Munc131 role in the process. Methods: Microdissection and extraction to obtain rat embryonic development 12.5d (E12.5) , E15.5, E18.5, newborn rats after birth 21 d (P21) and adu