Rat proximal tubular single improved method of determination of sodium pump activity_0.docVIP

 PAGE \* MERGEFORMAT 11 Rat proximal tubular single improved method of determination of sodium pump activity Of: Lei Luo Qian Liu Hong Yu Ai-Dong Liu Plateau de [Abstract] Objective: To Doucet described method, based on improving the determination of renal proximal tubule Na + K + ATPase activity. Methods: First, the use made #8203;#8203;of glass the minute hand can be accurately and quickly isolated from proximal tubules, followed by , better performance and ease of use of conductive foil instead of folded aluminum, simplifying the process of incubation and determination. Results: Improved methods and Doucet established classical methods, the determination was no significant difference (Pgt; 0.05), but small tube length to determine the mean time-consuming and the transfer of incubation solution containing 32P significantly reduced the average time-consuming. Conclusion: This paper describes the determination of rat kidney proximal tubule Na + K + ATPase activity of improved methods to reduce the steps to shorten the operation time and exposure to radioactive environment of the time, simplify and improve the operating conditions, reducing the experimental requirements and cost. [Keywords:] proximal tubule; sodium pump; activity assay; improved methods; rat Tubular basement membrane sodium pump, is a function of the kidney to achieve its key enzyme, determination of enzyme activity under different conditions of kidney function is significant. Determination of renal tubular sodium pump activity of a single method, Doucet First, in 1979, a more detailed report [1] (referred to Doucet method, the method is still the determination of renal tubular sodium pump activity in the most accurate and commonly used method, especially in determination of the different segments of renal tubule sodium pump activity [ 2]. Over the past twenty years, Doucet has been some improvement method [3,4], but the improvement is not significant. Doucet law technically demandin