Rhubarb anthraquinone-induced diarrhea and its mechanism of preliminary study.doc

 PAGE \* MERGEFORMAT 13 Rhubarb anthraquinone-induced diarrhea and its mechanism of preliminary study 【Abstract】 Objective To compare the combination of rhubarb anthraquinone, free anthraquinone-induced diarrhea and to explore the role of the strength of related mechanisms. Methods fed with a high concentration of milk powder solution to create a high nutritional environment, in this study based on the combination of rhubarb anthraquinones and free anthraquinone-induced diarrhea effects in mice; and the use of factorial design analysis of milk powder-induced diarrhea in these the role of the role, and also inspected the combination of rhubarb and free anthraquinone anthraquinones in vitro pepsin activity. The results of free rhubarb anthraquinones 500,1 000 mg / kg could stomach contents of mice and a significant increase in small intestine contents; rhubarb combination of anthraquinone 500,1 000 mg / kg also significantly increased the weight of the contents of the small intestine in mice, but the pairs of mice influence the weight of stomach contents of weak; the drug group of mice can be a significant increase in protein concentration within the intestines. Factorial design experiments showed that both free anthraquinone or a combination of rhubarb anthraquinones can be significantly increased in normal mice (without using milk powder) in the stomach, small intestine and the entire gastrointestinal tract contents and protein content, and can significantly reduce the mouse small intestine, colon Cl-concentration and increased the concentration of mouse colon K; milk powder itself also has the similar role of rhubarb anthraquinones, with combination can enhance the role of rhubarb anthraquinones. In vitro experiments showed that both free anthraquinone or a combination of rhubarb anthraquinones on the activity of pepsin had no significant effect. Conclusion Rhubarb and integration of free anthraquinone anthraquinone all have a significant effect of di