Rhubarb insects on the drug containing serum on cultured human uterine leiomyoma cell proliferation.doc

 PAGE \* MERGEFORMAT 12 Rhubarb insects on the drug containing serum on cultured human uterine leiomyoma cell proliferation [Abstract] Objective To observe the different doses of rhubarb with worm on the drug serum on primary cultured human uterine leiomyoma cell proliferation. Methods and the establishment of primary cultured human uterine leiomyoma cell lines, the use of serum pharmacology method of processing cells to MTT colorimetric assay containing serum effect on uterine leiomyoma cells 12,24,48,72 h the optical density values #8203;#8203;based on cell survival. Results of rhubarb on drug serum containing insects can directly inhibit the proliferation of uterine leiomyoma cells. Conclusion Rhubarb on drug serum containing insects can directly inhibit cell proliferation of uterine fibroids, uterine leiomyoma cells in insect rhubarb play on drug treatment of serum containing the target cells. [Keywords:] Rhubarb; insects; uterine fibroids; cell culture; containing serum Abstract: ObjectiveTo observe the effects of different dosages of medicated sera of Dahuang and Zhechong on the proliferation of human leiomyoma cells in vitro.MethodsHuman leiomyoma cells were cultured in vitro.Treated the cells with Chinese herbs serum pharmacology method.The MTT assay was adopted to investigate the survival rate of 12 h, 24 h, 48 h, 72 h after the cells were treated.ResultsSerum containing Dahuang and Zhechong showed an direct inhibitory action on proliferation of uterine leiomyoma cells.ConclusionDahuang and Zhechong medicated serum has inhibitory effect on proliferation of hysteromyoma cells, which indicates hysteromyoma cells are target cells. Keywords:: Dahuang, Zhechong; Leiomyoma; Cell culture; Medicated serum Uterine fibroids (myoma of uterus), also known as uterine fibroids (leiomyoma of uterus), female genital mutilation is the most common benign tumor, but also one of the most common cancer in the body, which occurs in women of reproductive age,