Ruthenium (Ⅱ ) Complexes Synthesis and study of DNA bonding.doc

 PAGE \* MERGEFORMAT 11 Ruthenium (Ⅱ ) Complexes Synthesis and study of DNA bonding Author: Huang Mei, Liu Jun, He Li-Xin, Zhao Hao-Jie, Wen-Jie Mei ‘ [Abstract] Objective and methods of synthesis of a new ligand HPIA and its ruthenium (Ⅱ ) polypyridine complexes [Ru (bpy) 2 (HPIA)] ClO4 (bpy = 2,2 ‘ bipyridine, HPIA = 2  ( 2  hydroxyphenyl) imidazo [4, 5  f] acenaphthylene), with elemental analysis, electrospray mass spectrometry, fast atom bombardment mass spectrometry, NMR characterization of ligands and complexes; using electronic absorption spectra and viscosity Testing Research Complexes with DNA, mode of action. Results and Conclusion complexes [Ru (bpy) 2 (HPIA)] ClO4 and the role of DNA binding by inserting between. [Keywords:] ruthenium (Ⅱ ) complexes; DNA; viscosity testing; bonding Abstract: A new ligand HPIA (2  (2  hydroxyphenyl)  imidazo [4,5  f] acenaphylene, bpy = 2,2 ‘ bipyridine) and its ruthenium (II) complex [Ru (bpy) 2 (HPIA) ] ClO4 have been synthesized and characterized by elemental analysis, mass spectra and 1HNMR. The electrochemical behaviors of the complex were investigated by cyclic voltammetry. The DNA  binding properties of the complex were studied by spectroscopic methods and viscosity measurement. The experimental results indicate that the complex [Ru (bpy) 2 (HPIA)] ClO4 interacts with calf thymus DNA (CT  DNA) by intercalative mode into the base pairs of DNA. Keywords:: [Ru (bpy) 2 (HPIA)] ClO4; DNA; viscosity measurement Nucleic acid is an important component of organisms substance, which contains genetic information and to participate in these cells the expression of information, which led to metabolic processes and control the process. In recent years, studies of transition metal compounds, small molecules interact with DNA, to become very active in the field of bio-inorganic chemistry topics [1], the research aimed at finding the secondary structure of DNA probes and DNA photocleavage reagents.