Rosa laevigata on experimental gA nephropathy expression of MCP 1mRNA.doc

 PAGE \* MERGEFORMAT 17 Rosa laevigata on experimental gA nephropathy expression of MCP 1mRNA Author: Huang Yan SHU Yu-Yan Wei Yulan 【Abstract】 Objective To study the water-alcohol extract of Rosa laevigata experimental Ⅰ gA nephropathy renal tissue MCP  1mRNA expression, understanding of its mechanism. Methods Ⅰ gA nephropathy rat model, 36 animals were randomly divided into normal group, model group and treatment group, with RT-PCR method to detect the experimental group, MCP  1mRNA expression in kidney tissue. Results MCP  1mRNA in the three experimental groups in the relative expression levels, respectively: (0.308 3 ± 0.159 4), (0.873 2 ± 0.501 7), (0.418 2 ± 0.193 3), compared with model group, Rosa laevigata the treatment group can effectively inhibit MCP  1mRNA expression in kidney tissues (P lt;0.05). Conclusion Rosa laevigata can down-regulate MCP  1mRNA expression in the kidney to reduce the local inflammatory response, reducing kidney damage, protect the kidneys. Keywords: Rosa laevigata Ⅰ gA nephropathy monocyte chemoattractant protein factor Abstract: ObjectiveTo study the effects of Rosa laevigata Michx on expression of MCP  1mRNA in rat experimental Ⅰ gA nephropathy model renal tissue at level of gene which can help to understand mechanism of action. MethodsAfter IgA nephropathy model was established, 36 rats were divided at random into normal group, model group and treatment group. The expression of MCP  1mRNA in renal tissue by RT-PCR methods. ResultsThe expression of MCP-1mRNA in three groups were (0.308 3 ± 0.159 4), (0.873 2 ± 0.501 7) , (0.418 2 ± 0.193 3) respectively. Compared with model group, Rosa Laevigata Michx could inhibit the expression of MCP  1mRNA `in renal tissue (P lt;0.05). ConclusionRosa laevigata Michx can decrease the expression of MCP  1mRNA in kidney, diminish local inflammatory reaction, relieve renal loss and protect renal function. Keywords:: Rosa laevigata Michx; Ⅰ gA nep