Rutin and quercetin FlosSophorae Determination of progress.doc

 PAGE \* MERGEFORMAT 22 Rutin and quercetin FlosSophorae Determination of progress Study: CAO Xiao Wang Lijun Niu steel in the first flower [Keywords:] Summary Determination of quercetin and rutin from bud Bud of the main active ingredient is flavonoids rutin and quercetin and other substances. Rutin has a wide range of pharmacological activity, to maintain and restore the normal elasticity of the capillary, to enhance its resistance to prevent blood cells gather, for the prevention and treatment of clinical stroke , hypertension and other cardiovascular diseases [1], quercetin has anti-inflammatory, antioxidant, anti-allergic, antibacterial, antiviral, and many other pharmacological effects [2]. China is the exporter of rutin, a bud of main raw material for extraction of rutin, 2005 Edition lt;lt;Chinese Pharmacopoeiagt; “(a) provides that the content of total flavonoids in Sophora japonica (anhydrous rutin) not less than 20%, anhydrous Rutin (C27H30O16 ) not less than 15% [3]. Therefore, the study of rutin and quercetin content of bud of test methods on quality control of bud of a very important significance. now bud of the determination of rutin and quercetin methods are summarized below. A spectrophotometric 1.1 UV absorption spectrophotometry, etc. Lee et al [4], etc. Simultaneous Determination of UV absorption of rutin and quercetin in Sophora japonica. Quercetin and rutin were prepared one-component standard solutions, determine their wavelength of 240 ~ 380 nm in the absorbance degree, after roughing and fine selection, respectively, such as Quercetin and find out the absorption wavelength of λ1247, λ2266, λ3340, λ4370. and then prepared a series of concentrations were rutin and quercetin in solution, measured at a wavelength of 247 nm and 266 nm, wavelength of 340 nm and absorbance at 370 nm to calculate the corresponding absorbance difference, ΔA-C respectively and the linear regression equation of calibration curve. determined by th