Secondary TLC - Fluorescence Determination of hesperidin contents of Effects of Peikun Capsule.docVIP

 PAGE \* MERGEFORMAT 6 Secondary TLC - Fluorescence Determination of hesperidin contents of Effects of Peikun Capsule Abstract A Effects of Peikun Capsule secondary hesperidin TLC - fluorescence analysis. That is first used ethyl acetate - methanol - water (100:17:13) started, and then with toluene - ethyl acetate - methanol - water (20:10:1:1) upper solution started; fluorescence conditions λ ex = 310nm , λ em = 510nm. The method can effectively eliminate the interference of coexisting components, and to improve detection sensitivity. Hesperidin in the range of 1μ g ~ 11μ g good linearity, r = 0.9977. Keywords:: Effects of Peikun Capsule; hesperidin; the second TLC; fluorescence spectrophotometry Effects of Peikun Capsule is based on the ancient Fang Peikun pills from Angelica, Astragalus, Eucommia ulmoides, Atractylodes, Citrus, etc. made of 21 Chinese herbal medicines, there are supplement qi and blood, liver and kidney and so the effectiveness of Zi. In order to better control its quality, we have established a second thin-layer chromatography - fluorescence method for determination of hesperidin in which content. An instrument and reagent 1.1 Instrument ATTD UV lamps; RF-540 fluorescence spectrophotometer (Shimadzu); TGL-16C high-speed centrifuge (Shanghai). 1.2 reagent of hesperidin reference substance (Chinese medicines and biological products); silica gel G (Qingdao Marine Chemical Plant); Effects of Peikun capsule samples (Xian Chia Tai Pharmaceutical Co., Ltd., batch number 971101,971102,971103). 2 Methods and Results 2.1 Reference Standards and test products for the preparation of the solution to take appropriate reference substance of hesperidin, using methanol as a reference substance solution made of 0.61mg/ml solution. Another Weigh Effects of Peikun Capsule contents 3g, buy conical flask, add methanol 20ml ultrasonic treatment 30min, filtration, and then methanol, washed twice, and combined filtrate concentrated to 10ml capacity and is s