Selenium Senecio grass and selenium polysaccharide content.docVIP

 PAGE \* MERGEFORMAT 15 Selenium Senecio grass and selenium polysaccharide content [Abstract] Objective To determine the selenium Senecio polysaccharide grass. Methods reflux extraction and ultrasonic synergistic microwave extraction of selenium polysaccharide extracted samples, the use of orthogonal ultrasonic synergistic extraction of microwave extraction conditions best, with sulfuric acid - Senecio anthrone method for the determination of selenium polysaccharide content of the grass, using graphite furnace atomic absorption determination of selenium in selenium polysaccharide. The results reflux extraction the extraction of crude polysaccharide content of 5.100% Se, the recovery of 97.83%, precision 0.95% Microwave and Ultrasonic synergistic extraction the extraction of crude polysaccharide content of 5.395% Se, recovery 97.93%, precision 1.170%, graphite furnace atomic absorption spectrometry crude selenium content of selenium polysaccharide 24.4g / g, recovery 97.64%, precision degree of 1.901%. Conclusion synergistic extraction of microwave ultrasonic extraction of Se Senecio grass was higher than reflux extraction of polysaccharide extracted from the grass back to the soul of selenium polysaccharide content, therefore, Microwave and Ultrasonic synergistic extraction is a rapid, sensitive method for the extraction . [Keywords:] Senecio grass, selenium polysaccharide, graphite furnace atomic absorption spectrometry, selenium Mama Senecio Senecio cannabifolius Lessl. Also known as broad leaf grass back to the soul, for the Compositae Senecio perennial herb, is mainly distributed in Northeast China, Hebei and other places, and North Korea, Japan, the former Soviet Union, Far East, growing in the ravine forest edge and wet meadow, the plant has a stasis, stop bleeding, the efficacy of pain [1], are widely used in the civil cold, inflammation of the treatment. Senecio plant contains pyrrolizidine alkaloids, such alkaloids metabolites in vivo liver an